Mmp 9 Antibody Cell Signaling

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding phe542 of human mmp 9 protein.

Mmp 9 antibody cell signaling.

Incubate with rotation for 1 3 hr at 4 c. One mechanism of mmp regulation is transcriptional 5. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lys115 of human mmp 9. Lane 1 is 10 input lane 2 is rabbit da1e mab igg xp isotype control 3900 and lane 3 is mmp 2 d4m2n rabbit mab.

Once synthesized mmp exists as a latent proenzyme. Incubate with rotation overnight at 4 c. 2001 curr opin cell biol 13 534 40. Anti rabbit igg hrp linked antibody 7074 was used as secondary antibody.

Background the matrix metalloproteinases mmps are a family of proteases that target many extracellular proteins including other proteases growth factors cell surface receptors and adhesion. Matrix metalloproteinase 9 92 kda gelatinase 92 kda type iv collagenase gelatinase b mmp9 human. Antibodies are purified by protein a and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lys554 of human mmp 2 protein.

Western blot analysis was performed using mmp 2 d4m2n rabbit mab as primary antibody. Mmp 13 is synthesized as a latent proenzyme and proteolytic removal of the inhibitory propeptide domain is required for enzyme activation. It was detected by western blot in total lysate of human primary leukemia blasts. Microcentrifuge for 30 sec at 4 c.

Background the matrix metalloproteinases mmps are a family of proteases that target many extracellular proteins including other proteases growth factors cell surface receptors and adhesion. Immunoprecipitation of mmp 2 protein from u 118mg cell extracts. Maximum mmp activity requires proteolytic cleavage to generate active mmps by releasing the inhibitory propeptide domain from the full length protein 5. Mmp 9 is a matrix metalloprotease produced from immune cells including macrophages and neutrophils which plays important roles in wound healing immunity and angiogenesis.

Mmp9 in a mouse brain tumor model 1 write a review. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence based around amino acid residue gly657 of human mmp 9. In this product review i report results that the anti mouse mmp 9 antibody from cell signaling showed unsurpassed performance in detecting the protein in both western blotting and immunofluroscence microscopy. Antibodies are purified by protein a and peptide affinity chromatography.

Add primary antibody at the appropriate dilution as recommended in the product datasheet to 200 µl cell lysate at 1 mg ml.